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Tobacco acid pyrophosphatase
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Tobacco acid pyrophosphatase : ウィキペディア英語版
Tobacco acid pyrophosphatase

Tobacco Acid Pyrophosphatase (TAP) is an enzyme that catalyses the hydrolysis of a phosphoric ester bond in a broad spectrum of molecules, including the 5'-end of mRNA.
During mRNA maturation the 5' triphosphate of the new mRNA molecule is rapidly removed. The diphosphate 5' end then attacks the α-phosphorus atom of a methylated GTP to form a very unusual 5'-5' triphosphate linkage, called ''cap''.〔(Online version )〕 In molecular biology, TAP is used to hydrolyse a phosphodiester bond of this particular structure and release a mRNA molecule with only one phosphate group in the 5'-end, for instance in protocols for RACE (Rapid Amplification of cDNA Ends).
== References ==


抄文引用元・出典: フリー百科事典『 ウィキペディア(Wikipedia)
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